The development of whole-genome microarrays for Arabidopsis and poplar has led
to the identification of hundreds of new wood-associated genes, which, in
turn, has facilitated reverse genetics and in vitro experiments revealing
protein-protein and protein-DNA interactions that dramatically influence wood
formation. Such results illustrate the value of integrating transcriptomics
with interactomics to identify regulatory networks important to the production
of woody biomass. We are identifying protein-protein interactions relevant to
genes expressed in wood-forming tissues of Populus trichocarpa.
Objectives and Progress
List of objectives from proposal and progress.
Rigorous Yeast 2-Hybrid Screen
Diagram of steps in screening cDNA library.
Xylem-Active Gene Interactions
The genes investigated here with Y2H interactions, sequences, primers, etc.
Contact Information
The poplar biomass interactome project is a collaboration among
the Department of Forest Resources and Environmental Conservation,
the Department of Horticulture, and the Virginia Bioinformatics Institute at Virginia Tech.
Publications
Journal articles generated by the poplar biomass interactome project.
Funding
This research was
supported by the Office of Science (BER), U.S. Department of Energy, Grant
No. DE-FG02-07ER64449 as part of
Plant Feedstock Genomics for Bioenergy, a joint program of the U.S. Department of Energy and U.S. Department of Agriculture.
